Description
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TNC. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for TNC. The competitive inhibition reaction is launched between with pre-coated TNC and TNC in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of TNC in the samples. The color development is stopped and the intensity of the color is measured